|
KMID : 1161420150180040460
|
|
Journal of Medicinal Food
2015 Volume.18 No. 4 p.460 ~ p.467
|
|
|
Antioxidant, Antimutagenic, and Anticarcinogenic Effects of Papaver rhoeas L. Extract on Saccharomyces cerevisiae
|
|
Todorova Teodora
Pesheva Margarita
Gregan Fridrich
Chankova Stephka
|
|
|
Abstract
|
|
|
|
The aim of this work was to analyze the antioxidant and antimutagenic/anticarcinogenic capacity of Papaver rhoeas L. water extract against standard mutagen/carcinogen methyl methanesulfonate (MMS) and radiomimetic zeocin (Zeo) on a test system Saccharomyces cerevisiae. The following assays were used: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, quantitative determination of superoxide anion (antireactive oxygen species [antiROS test]), DNA topology assay, D7ts1 test?for antimutagenic?and Ty1 transposition test?for anticarcinogenic effects. Strong pro-oxidative capacity of Zeo was shown to correlate with its well-expressed mutagenic and carcinogenic properties. The mutagenic and carcinogenic effects of MMS were also confirmed. Our data concerning the antioxidant activity of P. rhoeas L. extract revealed that concentration corresponding to IC50 in the DPPH assay possessed the highest antioxidant activity in the antiROS biological assay. It was also observed that a concentration with 50% scavenging activity expressed the most pronounced antimutagenic properties decreasing Zeo-induced gene conversion twofold, reverse mutation fivefold, and total aberrations fourfold. The same concentration possessed well-expressed anticarcinogenic properties measured as reduction of MMS-induced Ty1 transposition rate fivefold and fourfold when Zeo was used as an inductor. Based on the well-expressed antioxidant, antimutagenic, and anticarcinogenic properties obtained in this work, the P. rhoeas L. extract could be recommended for further investigations and possible use as a food additive.
|
|
|
KEYWORD
|
|
|
antiROS, corn poppy, D7, DNA topology, DPPH, MMS, scavenging activity, Ty1 transposition, yeast, zeocin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|